Viral Penetration Testing

What is a Viral Penetration Test?

The viral penetration test is a test method used to measure the resistance of various materials used in protective clothing to penetration by blood-borne pathogens. This test uses Phi-X174 as a test organism. The results are recorded as pass/fail.

Applicable Viral Penetration Standards:

ASTM F1671-22
AAMI PB70
ISO 16604
NFPA 1951
NFPA 1971
NFPA 1990
NFPA 1999

Sample Viral Penetration Specifications:

Each test specimen must be 3 x 3 inches (75mm X 75mm). Number of samples required for testing depends on the standard to which the material is being tested.

Viral Penetration Test Procedure Overview:

Specimens are prepared and conditioned according to the standard being performed. After samples are conditioned, they are placed into the test cells. The test cells are then filled with approximately 60 mL of the Phi-X174 challenge solution maintained at a concentration of at least 1.0 X 108 plaque forming units/ mL (PFU/mL). An external air source provides a set amount of pressure according to the standard being performed. After the completion of the test samples are assayed. The assay is then plated and incubated. Following incubation the plates are observed for plaques.