Viral Analysis and Characterisation

Viral vector vaccine characterization services including stability and aggregation testing from Intertek's Class I and Class II Sample Handling Facility

Recombinant viruses can be utilized as tools in the development of vaccines. Acting as a vector, they deliver an immunogenic protein from an infectious agent in order to enhance the immune response in the subject, or as in some cases, they are used where giving the actual agent as a vaccine would cause disease.

    Viral Vector Vaccine Stability and Aggregation Testing

    A common problem in the management of viral material is the tendency for aggregation and particle formation upon extended storage. Analytical techniques are required to study the propensity of solutions to form aggregates and to allow the investigation of the conditions encountered during manufacture and storage.

    Our teams of specialists apply techniques such as size exclusion chromatography with multi-angle laser light scattering (SEC-MALLS), also known as gel-permeation chromatography (GPC), and Dynamic Light Scattering (DLS) to study aggregation states of live viral products as part of stability trials.

    Our laboratories have supported vaccine developers and manufacturers over the last 20 years through provision of advanced characterisation programs. Our experts have worked on multiple studies involving these types of virus products; Adenovirus, Adeno-Associated Virus (AAV) and Lentivirus. With specialist laboratory facilities, we can handle Class I and Class II Biological Agents, as stated within the European Community Directive - Classification of Infectious Biological Agents by Risk Group (2000/54EC). The facility has been designed to handle recombinant genomic materials for the purposes of research.

    Viral Characterization Capabilities:

    • Liquid Chromatography (LC) and Liquid Chromatography - Mass Spectrometry (LC-MS) studies on whole virus species to profile the viral proteome
    • Viral proteome fingerprinting by chromatography (HPLC), MALDI Mass Spectrometry and gel electrophoresis (SDS-PAGE). MALDI is useful for proteins up to around 150 KDa.
    • Separation of empty capsids from intact viral particles by anion exchange chromatography
    • Protein composition of empty capsids by HPLC
    • Digestion of isolated proteins followed by LCMS and / or MALDI-MS to give detailed information to assist identification of viral proteins
     
    Need help or have a question? +1-858-558-2599
     

    Need help or have a question?

    +1-858-558-2599
    EMEA UK:
    +44 161 721 5247