Artificial Lesion Preparation for In Vitro Remineralisation and Demineralisation Studies

Artificial lesion preparation to support in vitro studies of tooth remineralisation & demineralisation and study of acid induced lesions/white-spot lesions

Artificial lesion preparation is the initial stage of in vitro oral care product studies to assess tooth remineralisation or demineralisation. Lesions are areas of decalcified tooth enamel which may develop into dental caries or be precursors to dental erosion.

The relative efficacy of dentifrices, such as toothpastes, to repair of demineralised tooth enamel is often compared using in vitro models and laboratory testing. Fluoride is often the active agent of choice in such dentifrices though other agents may also assist in enamel repair. In vitro remineralisation and demineralisation studies are a cost-effective alternative to clinical trials which can guide formulation development and support product claims.

Our experts can provide artificial lesion preparation to support in vitro studies of enamel remineralisation.  We are able to provide you with lesions so that you can carry out your own in vitro studies. Alternatively we can design and carry out bespoke studies that meet your particular requirements, whether they are for toothpastes, mouthrinses, gels or other type of dentifrice.

Within our dedicated oral care testing laboratory we can produce artificial lesions in bovine or human enamel or dentine in a wide variety of shapes and sizes. Lesion ‘windows’ can be created by protecting the sides and undersides of the substrates with dental wax or protective coatings. We are also able to provide you with thin cross-sections for analysis by microradiography.

Our Total Quality Assurance expertise in developing robust protocols or adopting literature-sourced protocols means we can tailor the protocol to your specific product application instructions. We can produce quick and simple acid-induced lesions or alternatively produce lesions over a period of several days by incubation in acid gels. These can then be used in enamel remineralisation models (pH cycling) and be measured using surface microhardness or microradiography.

For over 18 years our Clinical Research Services group has been a trusted partner for developers and manufacturers of oral care products. We provide a one-source solution for in vitro studies providing robust data to support the efficacy and safety of novel treatments enabling our customers to power ahead safely.

 
Intertek Clinical Research Services (CRS) has a dedicated in vitro laboratory which conducts testing of all aspects of Oral Care products. We are particularly adept in designing bespoke in vitro models to cater for your specific requirements. Intertek Clinical Research Services is licensed to handle and store human tissue under the Human Tissue Act 2004 and follows the principles outlined within the act as well as complying with local ethical guidelines.
Our team of laboratory scientists have a combined experience of over 30 years of testing oral care products and between them have degrees/PhD/MSc in Dentistry, Chemistry, Analytical Chemistry and Biological Sciences. They have also contributed to a variety of scientific papers in collaboration with many well-known clients and have presented posters at International scientific conferences.
 

Oral care product trial services:

Need help or have a question? +44 (0) 151 347 4810
 

Need help or have a question?

+44 (0) 151 347 4810
Wirral (UK):
+44 (0) 151 347 4810