In vitro dentinal tubule blockage studies supporting oral care product development and claims against tooth sensitivity
In vitro dentinal tubule blockage studies provide invaluable information to support the development of oral care products that protect against dentine hypersensitivity. Dentine lies beneath the enamel in our teeth and is characterized by a network of microscopic tubules, which extend through to the pulp chamber and its supply of nerves. External stimuli (heat, cold, pressure) on exposed dentine can trigger the nerves causing dentinal hypersensitivity.
Dentine hypersensitivity can be alleviated by physically blocking the tubules using specially-formulated toothpastes. The relative efficacy of different toothpastes often requires rapid evaluation in order to guide development of new and existing formulations cost-effectively. The degree of blockage can be assessed in vitro by brushing specimens of human dentine with paste slurry and then subjectively grading Scanning Electron Microscope (SEM) images of the surface.
Our oral care Laboratory team is experienced in carrying out in vitro dentinal tubule blockage studies to support the development of your toothpastes, medical devices and other products to address hypersensitivity.
Within our dedicated oral care lab we can produce dentine discs and blocks (bovine or human) in a variety of shapes and sizes using our range of abraders, wafering saws, lapping and polishing machines. The dentine is acid-etched to remove the smear layer and open up the tubules. Several samples are then assigned to each treatment group and can be brushed manually using an electric toothbrush together with your specified toothpaste(s). After each successive day of brushing samples can be examined under a scanning electron microscope (SEM) and images can be recorded for subjective analysis.
Images are graded by trained and calibrated assessors on a scale of 1 to 5 for the degree of occlusion. Means are calculated for each group of samples and results are statistically compared and presented in a comprehensive report that includes graphs, tables, data and images.
For over 18 years our Clinical Research Services group has been a trusted partner for developers and manufacturers offering Total Quality Assurance expertise of oral care products. We provide a one source solution for both in vitro studies and human trials providing robust data to support the efficacy and safety of novel treatments.