In Vitro Dentinal Tubule Blockage Studies

In vitro dentinal tubule blockage studies supporting oral care product development and claims against tooth sensitivity

In vitro dentinal tubule blockage studies provide invaluable information to support the development of oral care products that protect against dentine hypersensitivity. Dentine lies beneath the enamel in our teeth and is characterized by a network of microscopic tubules, which extend through to the pulp chamber and its supply of nerves. External stimuli (heat, cold, pressure) on exposed dentine can trigger the nerves causing dentinal hypersensitivity.

Dentine hypersensitivity can be alleviated by physically blocking the tubules using specially-formulated toothpastes. The relative efficacy of different toothpastes often requires rapid evaluation in order to guide development of new and existing formulations cost-effectively.  The degree of blockage can be assessed in vitro by brushing specimens of human dentine with paste slurry and then subjectively grading Scanning Electron Microscope (SEM) images of the surface.

Our oral care Laboratory team is experienced in carrying out in vitro dentinal tubule blockage studies to support the development of your toothpastes, medical devices and other products to address hypersensitivity.

Within our dedicated oral care lab we can produce dentine discs and blocks (bovine or human) in a variety of shapes and sizes using our range of abraders, wafering saws, lapping and polishing machines. The dentine is acid-etched to remove the smear layer and open up the tubules. Several samples are then assigned to each treatment group and can be brushed manually using an electric toothbrush together with your specified toothpaste(s). After each successive day of brushing samples can be examined under a scanning electron microscope (SEM) and images can be recorded for subjective analysis.

Images are graded by trained and calibrated assessors on a scale of 1 to 5 for the degree of occlusion. Means are calculated for each group of samples and results are statistically compared and presented in a comprehensive report that includes graphs, tables, data and images.

For over 18 years our Clinical Research Services group has been a trusted partner for developers and manufacturers of oral care products. We provide a one source solution for both in vitro studies and human trials providing robust data to support the efficacy and safety of novel treatments.

 
Intertek Clinical Research Services (CRS) has a dedicated in vitro laboratory which conducts testing of all aspects of Oral Care products. We are particularly adept in designing bespoke in vitro models to cater for your specific requirements. Intertek Clinical Research Services is licensed to handle and store human tissue under the Human Tissue Act 2004 and follows the principles outlined within the act as well as complying with local ethical guidelines.
Our team of laboratory scientists have a combined experience of over 30 years of testing oral care products and between them have degrees/PhD/MSc in Dentistry, Chemistry, Analytical Chemistry and Biological Sciences. They have also contributed to a variety of scientific papers in collaboration with many well-known clients and have presented posters at International scientific conferences.
 
Need help or have a question? +44 (0) 151 347 4810
 

Need help or have a question?

+44 (0) 151 347 4810
Wirral (UK):
+44 (0) 151 347 4810