Anti-Stain, Stain Removal and Tooth Whitening Testing

In vitro anti-stain, stain removal and tooth whitening testing to evaluate the performance of toothpastes, mouthwashes, strips, varnishes, gels & other dentifrices

Independent assessment of the tooth anti-stain or stain removal efficacy or teeth whitening benefits of new or developmental oral care product such as whitening toothpastes, stain control mouthwashes or whitening strips are key stages in their development.

Quantification of stain prevention, stain removal and whitening is often required to provide support for a wide range of tooth whitening products. Such data is often used in the generation of marketing claims or to guide further formulation development. 

Our dedicated team of oral care scientists conduct robust in vitro studies using relevant models for anti-stain, stain removal and whitening testing.  We are particularly adept at designing bespoke testing for your specific needs combining visual, instrumental and photographic assessments enabling our customers to power ahead safely.

A typical anti-stain study would measure in vitro stain build-up on human or bovine enamel specimens following multiple exposure to test product, saliva (human or artificial) and a specially formulated staining solution containing a mixture of tea, coffee, red wine and tobacco extract.  Relevant controls or benchmark products would be included and the test would be appropriately powered to reveal any significant product differences. Stain can be assessed subjectively by trained assessors or instrumentally using a chromameter that measures lightness and hue (L*a*b*). 


A typical stain removal study would involve staining human or bovine teeth or enamel specimens with a stain derived from e.g. tea and coffee and then measuring the amount of stain removal following one of several product applications.  In the case of toothpastes this could be performed using a mechanical brushing machine; we are also able to provide Pellicle Cleaning Ratio (PCR) testing whereby the abrasivity of toothpastes can be quantified against a calcium pyrophosphate standard. Stain is assessed before and after treatment using a chromameter that measures L*a*b* colour parameters.  

Whitening strips or peroxide-containing gels/varnishes that remove intrinsic tooth stain can be evaluated using extracted human teeth.  Results from all types of study are provided to the client in fully-signed off reports containing data tables, graphs, statistical analyses, discussion and conclusions.

For over 18 years our Clinical Research Services group has been a trusted partner for developers and manufacturers of oral care products. We provide a one source solution for both in vitro studies and stain removal and tooth whitening human trials providing robust data to support the efficacy and safety of novel treatments.

Intertek Clinical Research Services (CRS) has a dedicated in vitro laboratory which conducts testing of all aspects of Oral Care products. We are particularly adept in designing bespoke in vitro models to cater for your specific requirements. Intertek Clinical Research Services is licensed to handle and store human tissue under the Human Tissue Act 2004 and follows the principles outlined within the act as well as complying with local ethical guidelines.
Our team of laboratory scientists have a combined experience of over 30 years of testing oral care products and between them have degrees/PhD/MSc in Dentistry, Chemistry, Analytical Chemistry and Biological Sciences. They have also contributed to a variety of scientific papers in collaboration with many well-known clients and have presented posters at International scientific conferences.
Need help or have a question? +44 (0) 151 347 4810

Need help or have a question?

+44 (0) 151 347 4810
Wirral (UK):
+44 (0) 151 347 4810