Anti-stain, stain removal and tooth whitening laboratory testing to evaluate the performance of toothpastes, mouthwashes, strips, varnishes, gels & other dentifrices
Assessment of tooth anti-stain, stain removal or teeth whitening efficacy of novel oral care products such as whitening toothpaste, stain control mouthwashes or whitening strips can accelerate product development. This data is often used in the generation of marketing claims or to guide further formulation development.
Consumers are driving innovation in tooth whitening products and where performance can be clearly demonstrated through quantification of stain prevention, stain removal or whitening, brands can differentiate themselves in the marketplace.
In vivo clinical studies can be expensive to commission, with costs associated with screening and compensation of large numbers of suitable volunteers as well as the training of clinical and operations staff and running costs of clinical study sites and dental surgery facilities. Volunteers are subjected to moderately invasive dental procedures and may be requested to follow restrictions in terms of diet and dentifrice use over as many as 6 months which is a long time for a volunteer to remain compliant. Inevitably some volunteers will withdraw from the study and this must be factored in when deciding how many volunteers to enrol in the study.
Instead a well-designed in vitro or laboratory test can often yield the required data much more quickly and at a lower cost. Such studies – whether they are to support early-stage development of a product formulation or in support of marketing claims or pack claims - may be routinely carried out.
In Vitro Laboratory Studies for Anti-Stain, Stain Removal or Tooth Whitening
Our dedicated team of oral care scientists conduct robust in vitro studies using relevant models for anti-stain, stain removal, and whitening testing. We are particularly adept at designing bespoke testing for your whitening toothpaste, stain control mouthwash or whitening strip products and your specific testing needs combining visual, instrumental and photographic assessments enabling our customers to power ahead safely.
A typical anti-stain study would measure in vitro stain build-up on human or bovine enamel specimens following multiple exposure to test product, saliva (human or artificial) and a specially formulated staining solution containing a mixture of tea, coffee, red wine and tobacco extract. Relevant controls or benchmark products would be included and the test would be appropriately powered to reveal any significant product differences. Stain can be assessed subjectively by trained assessors or instrumentally using a chromameter that measures lightness and hue (L*a*b*).
A typical stain removal study would involve staining human or bovine teeth or enamel specimens with a stain derived from e.g. tea and coffee and then measuring the amount of stain removal following one of several product applications. In the case of toothpastes this could be performed using a mechanical brushing machine; we are also able to provide Pellicle Cleaning Ratio (PCR) testing whereby the abrasivity of toothpastes can be quantified against a calcium pyrophosphate standard. Stain is assessed before and after treatment using a chromameter that measures L*a*b* colour parameters.
Whitening strips or peroxide-containing gels/varnishes that remove intrinsic tooth stain can be evaluated using extracted human teeth. Results from all types of study are provided to the client in fully-signed off reports containing data tables, graphs, statistical analyses, discussion and conclusions.
For over 19 years our Clinical Research Services group has been a trusted partner for developers and manufacturers of oral care products. We provide a one source solution for both in vitro studies and stain removal and tooth whitening human trials as part of our wider in vitro oral care product laboratory testing, oral care clinical testing for personal care products and dental clinical trials providing robust data to support the efficacy and safety of novel treatments.