Anti-plaque and anti-calculus laboratory testing for oral care products to evaluate anti-bacterial, anti-adhesion or enzyme inhibition agents or crystal growth inhibition agents
In vitro anti-plaque and anti-calculus testing of oral care products can help to assess product performance. Oral care product manufacturers often incorporate anti-plaque or anti-calculus agents in their dentifrice formulations to provide obvious consumer benefits, in fact the majority of dentifrices and also some mouthwashes and flosses now contain one or more active anti-calculus agents to achieve so‐called tartar control. New anti-plaque and anti-calculus agents require screening using a simple and effective model that can be used to guide product development.
Laboratory Anti-plaque or Anti-calculus Activity Evaluation
We provide in vitro or laboratory screening of anti-plaque and anti-calculus activity using a model in which plaque is grown on roughened glass rods seeded with human saliva. Typically, the growth will be built up over a period of four to five days and will involve cycling the rods between product treatment, saliva, and nutrient broth. Anti-plaque benefits can be quantified in terms of dry biofilm weight and/or by measuring Total Viable Counts (TVC) of aerobic bacteria.
Our dedicated team of scientists can design the test according to your specific requirements and incorporate the analyses appropriate to your needs. We can also perform statistical analysis of the data which will be provided to you in a fully signed-off report.
Anti-calculus tests use a similar model of in vitro plaque growth which also involves periodic exposure to crystal growth inhibitors and plaque mineralising solution. At the end of the test the residual biofilm is dissolved in acid and tested for calcium content using inductively coupled plasma - optical emission spectroscopy (ICP-OES) which is conducted in a Good Clinical Practice (GCP) compliant laboratory. The effectiveness of different agents can thereby be compared.
For over 19 years our Clinical Research Services group has been a trusted partner for developers and manufacturers of oral care products. Our Total Quality Assurance expertise, brings quality and safety to life by providing a one-source solution for in vitro oral care product studies and dental clinical trials providing robust data to support the efficacy and safety of novel treatments.