In vitro anti-plaque and anti-calculus testing to evaluate anti-bacterial, anti-adhesion or enzyme inhibition agents or crystal growth inhibition agents
In vitro anti-plaque and anti-calculus testing can be carried out using essentially similar models whereby human plaque is grown on roughened glass rods seeded with whole human saliva.
Oral care product manufacturers often incorporate anti-plaque or anti-calculus agents in their dentifrice formulations to provide obvious consumer benefits. New agents require screening using a simple and effective model that can be used to guide product development.
We provide in vitro screening of anti-plaque and anti-calculus benefits using a model in which plaque is grown on roughened glass rods seeded with human saliva. Typically the growth will be built up over a period of four to five days and will involve cycling the rods between product treatment, saliva, and nutrient broth. Anti-plaque benefits can be quantified in terms of dry biofilm weight and/or by measuring Total Viable Counts (TVC) of aerobic bacteria. Our dedicated team of scientists can design the test according to your specific requirements and incorporate the analyses appropriate to your needs. We can also perform statistical analysis of the data which will be provided to you in a fully signed-off report.
Anti-calculus tests use a similar model of in vitro plaque growth which also involves periodic exposure to crystal growth inhibitors and plaque mineralising solution. At the end of the test the residual biofilm is dissolved in acid and tested for calcium content using ICP-OES. The effectiveness of different agents can thereby be compared.
For over 18 years our Clinical Research Services group has been a trusted partner for developers and manufacturers of oral care products. We provide a one source solution for both in vitro studies and human trials providing robust data to support the efficacy and safety of novel treatments.