We have in-depth expertise in GLP and cGMP structural characterization of proteins.
Structural Characterization and Confirmation:
Isoform and Impurity Studies using PAGE, SDS-PAGE, IEF, CE, HPLC
Amino Acid Composition by Ion Chromatography
Peptide and Disulphide Bridge Maps using MALDI or Electrospray MS
N- and C- Terminal Sequences by LC-MS/MS Sequencing of Peptide Fragments from Digests containing N- and C- Termini
Sequencing and Post Translational Modification Studies using a Broad Range of Digest Strategies and LC-MS/MS (including enhanced scan techniques)
Studies of Conjugated Products (e.g., Protein-Toxin Conjugates) using LCMS and LC-MS/MS to determine Loading and Binding sites
Glycosylation Studies by Enzymatic Glycan Cleavage and Fingerprinting by IC or MALDI-MS
Advanced Glycosylation Studies using Multiple Cycles of Selective Enzymatic Cleavage and MALDI-MS to build up a detailed picture of branching
Viral Proteome Studies to support the characterization of Viral Products used in Vaccine and Gene Therapy products
Basic Activity Assays e.g. Sandwich ELISA, Western-Blot
Request more information to see how Intertek can help your organization with Protein Structural Characterization and Confirmation.
